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cell surface marker expression fluorescein  (Thermo Fisher)


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    Structured Review

    Thermo Fisher cell surface marker expression fluorescein
    Cell Surface Marker Expression Fluorescein, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+surface+marker+expression/Fluorescein/pmc11115656-94-2-40
    Average 99 stars, based on 1 article reviews
    cell surface marker expression fluorescein - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Control:

    Article Title: An antisense oligonucleotide-based strategy to ameliorate cognitive dysfunction in the 22q11.2 Deletion Syndrome
    Article Snippet: Total RNA was extracted from HPC, PFC and Somatosensory Cortex (SSC) using the RNeasy Mini Kit (catalog#1038703, QIAGEN, Hilden, Germany) or using the miRVana miRNA isolation kit (#AM1560, Ambion, Thermo Fisher Scientific, Waltham, MA, USA) for RNA extraction of hiPSC and derived neurons samples in accordance to their manuals. cDNA was synthesized using High-Capacity RNA-to-cDNA Kit from Applied Biosystems (cat#4387406, Thermo Fisher Scientific Baltics, Vilnius, Lithuania). qRT-PCR was performed using the Bio-Rad CFX-384 qPCR instrument (Bio-Rad, Hercules, CA, USA) using TaqMan Universal Master Mix II, with UNG (catalog#4440038, Thermo Fisher Scientific Baltics, Vilnius, Lithuania). .. Mouse Gapdh Endogenous Control (cat# 4352339E, Life Technologies, Warrington, United Kingdom) served as housekeeping gene and TaqMan Mm01197551_m1 (catalog#4351372) probe for mouse Emc10 as well as Mm01208065_m1 for Emc10-1 (cat# 4351372) and Mm01197555_m1 for Emc10-2 (cat# 4351372) mRNA detection were used for the qRT-PCR assay. .. Mice were maintained at a temperature of 37 °C for the duration of the surgery using a water regulated heating pad (T/Pump TP 700, Stryker Corporation, Kalamazoo, MI, USA).

    Quantitative RT-PCR:

    Article Title: An antisense oligonucleotide-based strategy to ameliorate cognitive dysfunction in the 22q11.2 Deletion Syndrome
    Article Snippet: Total RNA was extracted from HPC, PFC and Somatosensory Cortex (SSC) using the RNeasy Mini Kit (catalog#1038703, QIAGEN, Hilden, Germany) or using the miRVana miRNA isolation kit (#AM1560, Ambion, Thermo Fisher Scientific, Waltham, MA, USA) for RNA extraction of hiPSC and derived neurons samples in accordance to their manuals. cDNA was synthesized using High-Capacity RNA-to-cDNA Kit from Applied Biosystems (cat#4387406, Thermo Fisher Scientific Baltics, Vilnius, Lithuania). qRT-PCR was performed using the Bio-Rad CFX-384 qPCR instrument (Bio-Rad, Hercules, CA, USA) using TaqMan Universal Master Mix II, with UNG (catalog#4440038, Thermo Fisher Scientific Baltics, Vilnius, Lithuania). .. Mouse Gapdh Endogenous Control (cat# 4352339E, Life Technologies, Warrington, United Kingdom) served as housekeeping gene and TaqMan Mm01197551_m1 (catalog#4351372) probe for mouse Emc10 as well as Mm01208065_m1 for Emc10-1 (cat# 4351372) and Mm01197555_m1 for Emc10-2 (cat# 4351372) mRNA detection were used for the qRT-PCR assay. .. Mice were maintained at a temperature of 37 °C for the duration of the surgery using a water regulated heating pad (T/Pump TP 700, Stryker Corporation, Kalamazoo, MI, USA).

    Expressing:

    Article Title: Pharmacological inactivation of a non-canonical gp130 signaling arm attenuates chronic systemic inflammation and multimorbidity induced by a high-fat diet
    Article Snippet: For hematopoietic stem cells, bone marrow cells were stained using BD StemflowTM Mouse Hematopoietic Stem and Progenitor Cell Isolation Kit (BD, 560492) following the manufacturer’s instructions. .. For skin stem cells, populations of interest were based on negative DAPI (Fisher Scientific, 5748) expression and surface marker expressions ITGA6 (Invitrogen, 12-0495-82), ITGB1(BioLegend, 102215), CD34 (Invitrogen, 11-0341-82), Sca1 (BioLegend, 122513) were used . .. Flow cytometry data was analyzed using FlowJo software.

    Marker:

    Article Title: Pharmacological inactivation of a non-canonical gp130 signaling arm attenuates chronic systemic inflammation and multimorbidity induced by a high-fat diet
    Article Snippet: For hematopoietic stem cells, bone marrow cells were stained using BD StemflowTM Mouse Hematopoietic Stem and Progenitor Cell Isolation Kit (BD, 560492) following the manufacturer’s instructions. .. For skin stem cells, populations of interest were based on negative DAPI (Fisher Scientific, 5748) expression and surface marker expressions ITGA6 (Invitrogen, 12-0495-82), ITGB1(BioLegend, 102215), CD34 (Invitrogen, 11-0341-82), Sca1 (BioLegend, 122513) were used . .. Flow cytometry data was analyzed using FlowJo software.

    Sequencing:

    Article Title: An antisense oligonucleotide-based strategy to ameliorate cognitive dysfunction in the 22q11.2 Deletion Syndrome
    Article Snippet: Sequence-based reagent , TaqMan Mm01197551_m1 ( Emc10 ) , Thermo Fisher , cat#4351372 , Mouse Emc10 qRT-PCR. .. Sequence-based reagent , TaqMan Mm01208065_m1 ( Emc10-1 ) , Thermo Fisher , cat4351372 , Mouse Emc10 qRT-PCR. .. Sequence-based reagent , TaqMan Mm01197555_m1 ( Emc10-2 ) , Thermo Fisher , cat#4351372 , Mouse Emc10 qRT-PCR.

    Article Title: An antisense oligonucleotide-based strategy to ameliorate cognitive dysfunction in the 22q11.2 Deletion Syndrome
    Article Snippet: Sequence-based reagent , TaqMan Mm01208065_m1 ( Emc10-1 ) , Thermo Fisher , cat#4351372 , Mouse Emc10 qRT-PCR. .. Sequence-based reagent , TaqMan Mm01197555_m1 ( Emc10-2 ) , Thermo Fisher , cat4351372 , Mouse Emc10 qRT-PCR. .. Sequence-based reagent , Mouse Gapdh Endogenous Control , Life Technologies , cat#4352339E , Housekeeping gene for mouse qRT-PCR.

    Article Title: An antisense oligonucleotide-based strategy to ameliorate cognitive dysfunction in the 22q11.2 Deletion Syndrome
    Article Snippet: Sequence-based reagent , qRT-PCR primers , Integrated DNA Technologies , N/A , See for sequences . .. Sequence-based reagent , TaqMan Mm01197551_m1 ( Emc10 ) , Thermo Fisher , cat4351372 , Mouse Emc10 qRT-PCR. .. Sequence-based reagent , TaqMan Mm01208065_m1 ( Emc10-1 ) , Thermo Fisher , cat#4351372 , Mouse Emc10 qRT-PCR.

    other:

    Article Title: Injury-specific pathways shape corneal nerve regeneration dynamics
    Article Snippet: L1cam , Mm00493049_m1.



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